Measurement
M_CLTC

Part:BBa_K5088507:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-07-31)


Measurement construct - CLTC promoter+5'UTR from T. kok-saghyz


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 249
    Illegal EcoRI site found at 1553
    Illegal XbaI site found at 1609
    Illegal XbaI site found at 1635
    Illegal XbaI site found at 3448
    Illegal PstI site found at 3875
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 249
    Illegal EcoRI site found at 1553
    Illegal PstI site found at 3875
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 249
    Illegal EcoRI site found at 1553
    Illegal BglII site found at 924
    Illegal BglII site found at 1612
    Illegal BglII site found at 3451
    Illegal XhoI site found at 2695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 249
    Illegal EcoRI site found at 1553
    Illegal XbaI site found at 1609
    Illegal XbaI site found at 1635
    Illegal XbaI site found at 3448
    Illegal PstI site found at 3875
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 249
    Illegal EcoRI site found at 1553
    Illegal XbaI site found at 1609
    Illegal XbaI site found at 1635
    Illegal XbaI site found at 3448
    Illegal PstI site found at 3875
    Illegal NgoMIV site found at 2923
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3137


Design Notes

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Source

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References